Biotechnology Annual Review: 1

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    Find all citations in this journal default. Or filter your current search. Biotechnology Annual Review [01 Jan , Abstract Tetrazolium salts have become some of the most widely used tools in cell biology for measuring the metabolic activity of cells ranging from mammalian to microbial origin.

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    Cover image Biotechnology Annual Review Critical review and appraisal of published clinical literature: Useful skill in biotechnolog pp. 1– (). Biotechnology annual review | The "Biotechnology Annual Review" covers the The purpose of this chapter is twofold; (1) to re-examine the role of protein.

    With mammalian cells, fractionation studies indicate that the reduced pyridine nucleotide cofactor, NADH, is responsible for most MTT reduction and this is supported by studies with whole cells. The net positive charge on tetrazolium salts like MTT and NBT appears to be the predominant factor involved in their cellular uptake via the plasma membrane potential. However, second generation tetrazolium dyes that form water-soluble formazans and require an intermediate electron acceptor for reduction XTT, WST-1 and to some extent, MTS , are characterised by a net negative charge and are therefore largely cell-impermeable.

    Considerable evidence indicates that their reduction occurs at the cell surface, or at the level of the plasma membrane via trans-plasma membrane electron transport.

    The implications of these new findings are discussed in terms of the use of tetrazolium dyes as indicators of cell metabolism and their applications in cell biology. Read Article at publisher's site.

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    Find out more Title discontinued as of Latest chapters Editorial Abstract only Preface. Review article Abstract only The social network of a cell: Recent advances in interactome mapping.

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    Review article Abstract only Gene expression microarray data analysis demystified. Deep support for molecular biomedical research.

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    Description Product Info Reviews Loading Take me to the survey. The purpose is to have a forum in which general doubts about the processes of publication in the journal, experiences and other issues derived from the publication of papers are resolved. Chapter 3 Application of twodimensional protein gels in biotechnology. We use cookies to help provide and enhance our service and tailor content and ads.

    Review article Abstract only Potential implications of availability of short amino acid sequences in proteins: An old and new approach to protein decoding and design. Review article Abstract only Network models in drug discovery and regenerative medicine. Review article Abstract only Use of the cauliflower Or gene for improving crop nutritional quality.